Gene Editing Lab 3D

Master CRISPR technology: guide RNA gRNA design (20-nucleotide target specific DNA sequence), Cas9 enzyme (molecular scissors create double-strand breaks), repair mechanisms (NHEJ error-prone deletions, HDR precise template-based corrections), off-target minimization (computational design validation sequencing)

Understand gene editing applications: medicine (sickle cell cure $2-3M approved 2023, CAR-T cancer immunotherapy 80-90% remission, Leber blindness in-vivo editing), agriculture (drought-resistant wheat, disease-resistant rice, CRISPR non-GMO advantage), research (disease models knock-out genes, drug discovery acceleration)

Apply molecular biology techniques: cell culture editing (transfect cells electroporation lipid nanoparticles), delivery methods (viral vectors AAV lentivirus, non-viral safer), validation (Sanger sequencing confirm edits, NGS detect off-targets, functional assays verify phenotype)

Evaluate ethical considerations: germline editing (embryos inheritable changes, He Jiankui scandal 2018, international moratorium), therapy vs enhancement (disease treatment acceptable, designer babies problematic), access equity (cost $2-3M only wealthy, social inequality concerns)

Explore biotechnology careers: genetic engineer ($1500-3500/month design CRISPR experiments), research scientist ($2000-4000/month PhD publications), bioinformatics specialist ($1800-3500/month Python R analyze sequencing), clinical genetic counselor ($1500-3000/month advise patients interpret genetic tests)